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The present study was undertaken to evaluate the osteogenic effect of Cervi cornu pantotrichum (CCP) on bone marrow stromal cells (BMSCs) which showed differentiation towards the osteoblastic phenotype. Cells were exposed to different culture medium (dexamethasone group, CCPE group and control group). The activities of osteoblastic markers such as alkaline phosphatase (ALP), bone gla protein (BGP) and bone morphogenetic protein-2 (BMP-2) were detected by Elisa. Cell proliferation was measured by MTT assay. The bone nodule formation was measured with alizarin red stain. Data anlysis showed that CCPE had significantly promoted proliferation of BMSCs in the first three days and stimulated the BMSCs differentiate into osteoblast. Calcium nodule was found after 18days. This results demonstrate that CCPE has a stimulatory effect on bone formation and maight be a useful remedy for the treatment of bone diseases such as osteoporosis.
Cervi cornu pantotrichum, aqueous extract, Bone marrow stromal cells, bone formation