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Single Nucleotide polymorphisms (SNP) are the most common type of DNA sequence polymorphisms and of mutation in vertebrates. Keeping in view the involvement of Oxidised Low Density Lipioprotein Receptor 1 (OLR1) gene in lipid metabolism and the higher milk fat percentages in Bubalus bubalis, the present study was carried out to explore the SNPs in OLR 1 intron I in Mehsani and Banni breeds of buffalo by gene cloning and sequencing. Five SNPs at nucleotide positions viz. 327, 541, 957 and 1128 were detected and all the SNPs followed breed lineage. The SNP at positions 327 and 541 were found in Mehsana breed alone while as rest of the explored SNPs restricted to Banni breed of Bubalus bubalis. The frequency of each SNP was found to be 0.025.
Single Nucleotide Polymorphisms, buffalo, Oxidised Low density Lipoprotein Receptor 1.