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Short Communication Open Access
A new colorimetric method for the determination of thiamine hydrochloride is developed. It is based on the formation of coloured ion-pair complex between thiamine hydrochloride and alizarin brilliant violet R in the aqueous phase at pH 4.5 extractable into chloroform which obeyes Beer's law in the range of 2-16 mcg/ml.
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Author(s): P S S Prasad K P Rajasree K A Khan M Narayana Reddy