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Piperazine and its salts are reacted with alcoholic p-benzoquinone and buffered at pH 5.4 to give a coloured product with maximum absorption at 516 nm. The piperazine base has a molar absorptivity of 0.96 × 10 4 1/mol/cm, and Beer's law is obeyed in the range of 4-20 µg/ml. When applied to two commercial preparations, the proposed method gave mean recoveries within 1%, and relative standard deviation was less than 1%.