alexa Abstract | Comparative analysis of RAPD and ISSR marker assays for detecting genetic polymorphism in <i>Tilletia indica</i>

European Journal of Experimental Biology
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article Open Access

Abstract

Karnal bunt of wheat, caused by Tilletia indica Mitra is an important disease prevalent in several countries. The pathogen due to its heterothallic nature shows high variability. In the present study, genetic relationships among ten isolates of T.indica collected from different locations of India and 15 monosporidial lines raised from these isolates were investigated by using 34 RAPD and 28 ISSR primers. RAPD and ISSR primers revealed 92.82 and 98.4 % polymorphism, respectively. Similarity coefficient values ranged from 0.61 to 0.97 for RAPD and 0.61 to 0.96 for ISSR primers. The dendrogram developed by RAPD and ISSR primers based analysis grouped the isolates and monosporidial lines in different clusters. Mental test employed for detection of goodness of fit established cophenatic correlation value for both the primer systems and it was significant at P 0.01. Clustering of isolates within groups was also similar based on RAPD and ISSR derived dendrograms. In our study, both marker systems were similar except for the percentage polymorphism which was found to be greater using ISSR, thus indicating the greater effectiveness of ISSR primers for estimating genetic diversity of Tilletia indica.

To read the full article Peer-reviewed Article PDF image

Author(s): Shabana Parveen M S Saharan Ajay Verma and Indu Sharma

Keywords

Karnal bunt, Tilletia indica, Wheat, DNA polymorphism, Variability

 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us