alexa Abstract | Comparison of the effects of three commercial media on preimplantation mouse embryo development and morphological grading.

Biomedical Research
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)


Embryo culture is a vital procedure in Assisted Reproductive Technologies (ART). Assessment of embryonic morphology and development is of importance in the selection of embryos for transfer. Survivability of embryos in culture media depends on nutrient supply to maintain embryonic growth at every stage of preimplantation development. The objective of this study was to observe the effect of M16, HTF and KSOM commercial media on preimplantation embryonic development and morphological grading. Female mice were superovulated with pregnant mare’s serum gonadotropin (PMSG) followed by human chorionic gonadotropin (hCG) 48 h later. The oviducts were flushed at 23 -25 h post-hCG using M2 medium. Embryos with two pro-nuclei were selected for culture. A total of 240 embryos were equally divided for culture in M16, HTF and KSOM media. Embryonic development and morphological grading were observed at 48, 67, 74, 93 and 11 h post-hCG until the hatched blastocyst stage. Significantly higher numbers of blastocysts was observed in HTF and KSOM cultures at 93 h post-hCG, compared to M16 culture (p<0.05). HTF showed a significantly higher number of Grade B4 blastocyst (p<0.05), while KSOM showed a significantly higher number of Grade B1 blastocysts (p<0.05), compared to M16. Morphological quality appeared to be stage dependant. M16 medium showed significantly higher number of 8-cell stage (n=62) compared to HTF (p= 0.026). Our observations suggested that for ICR mouse embryos, M16 is effective in promoting precompaction stage development until the 8-cell stage, while HTF and KSOM were more effective in promoting post-compaction stage development, hence producing more blastocysts.

To read the full article Peer-reviewed Article PDF image | Peer-reviewed Full Article image

Author(s): MohdFazirul M NorAshikin MNK Kamsani YS Sharaniza AbRahim Norhazlin JMYWanHafizah WJ Razif D Froemming GRA and Kapitonova MY


Murine embryo, embryo culture, development, culture medium.

Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version