alexa Abstract | Comprehensive Microarray Analysis Identify Dysregulated MicroRNAs in Pressure Overload Affected Hearts

Translational Biomedicine
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article Open Access

Abstract

Background: MicroRNAs (miRNAs) are emerging as key regulators of cardiovascular development and disease, being involved in cardiac hypertrophy, fibrosis, remodeling and heart failure. Aortic stenosis is the most common valvular heart disease, and thus, we undertook a detailed analysis of differentially expressed miRNAs in this disease.

Methods and Findings: We set up a model of aortic stenosis by performing transverse aorta constriction (TAC) in adult mice resulting in a rapid increase in left ventricle pressure load, pronounced hypertrophy, initiated fibrosis, impaired ejection performance and dysfunctional diastole. Using miRNA microarray we then identified more than 300 differentially regulated miRNAs in the hearts of mice affected by aortic stenosis. Five of these were validated using qRT-PCR in which miR-21 and miR-208b were striking because of their substantial 2-4 fold increase at day 21 post TAC when compared with the control group. Furthermore, we identified three novel miRNAs (miR-24, miR-301a and miR-335) that were highly induced in transverse aorta constricted mice compared to SHAM operated mice. Interestingly, the increased expression of miR-24, miR-301a and miR-335 in TAC affected hearts were not found in an animal model of myocardial infarction.

Conclusion: These data suggests that the novel miRNAs are regulated in the heart specific to aortic stenosis, and may serve as biomarker candidates for future diagnosis, prognosis or treatment.

To read the full article Peer-reviewed Article PDF image | Peer-reviewed Full Article image

Author(s): Tilde VE Mikael Schneider Peiyong Zhai Maria BS Lina AK Ditte CA Junichi Sadoshima Soren PS

Keywords

Aorta stenosis, Transverse aorta constriction, Hypertrophy, Fibrosis, Echocardiography, MicroRNA, Computational Biology,Molecular Development Biology

 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords