alexa Abstract | Cost Effective Production of Cellulase using Nonsterile Fermentation Technique

Journal of Chemical and Pharmaceutical Research
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Abstract

The present work was aimed to develop in vitro cost effective nonsterile fermentation technique to enhance cellulase production and also its optimization for carbon and nitrogen source, pH, and temperature. Cellulose degrading novel strain of Paenibacillus was isolated from soil and characterized further using 16S r-RNA analysis (LPUANSHUL strain LPU001; accession number JQ916899). Optimum pH and temperature for cellulase activity was recorded 8.0 and 70o C respectively. Enzyme stability was noted at 60oC for up to 5 hours and activity was also evaluated in the presence of divalent cobalt, manganese and magnesium ions. Bushnell-Haas medium enriched with 0.5% CMC and beef extract showed the best enzyme activity. Optimization of substrate was done by using wheat straw under solid state fermentation (SSF). The enzyme isolated from solid state fermentation was analyzed in a zymogram

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Author(s): Anshul Joshi Neeta Raj Sharma and Mahiti Gupta

Keywords

Cellulase, Cellulolytic microorganisms, Saccharification, Solid state fermentation, Fermentation Technique

 
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