alexa Abstract | Cytotoxicity activity of rabbit antisera of riboflavin carrier protein (RCP) against HeLa cells, MCF-7 and A549 cancer cell lines

Journal of Chemical and Pharmaceutical Research
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Original Articles Open Access

Abstract

In the present study, riboflavin carrier protein was purified from hen and spotted owlet (Athene brama) egg yolk and white. To determine and compare in-vitro cytotoxic activity of Rabbit antiserum against Riboflavin Carrier Protein (RCP) isolated from white as well as yolk of spotted owlet egg and hen eggs on HeLa cells, MCF-7 and A549 cancer cells. The anticancer activity of Riboflavin Carrier proteins (RCP) purified from egg-white of spotted owlet (Athene brama) antiserum was 16.531 %, egg-yolk antiserum was 11.231 % on HeLa cell lines. Spotted owlet egg yolk antiserum on HeLa cells showed less cytotoxic potential. Riboflavin Carrier proteins (RCP) purified from egg-white of spotted owlet antiserum cytotoxic effect was 25.171 %, egg-yolk antiserum was 20.104% and Hen egg-white antiserum was 26.737 %, egg-yolk antiserum was 24.024% on A549 cancer cells. The anticancer activity of Riboflavin Carrier proteins (RCP) purified from egg white of spotted owlet (Athene brama) antiserum was 18.922 %, egg-yolk antiserum was 32.842 % and Hen egg-white antiserum was 22.499 %, egg-yolk antiserum was 18.860 % on Breast cancer cells. Highest cytotoxic activity was seen in spotted egg yolk antiserum on Breast cancer cell lines and standard drug used was Doxorubicin (48.528 % of Cytotoxicity). IC50 values for A549 were 12.46 ± 0.9 & 11.20 ± 0.8 for hen white, yolk and 11.64 ± 0.9 & 14.89 ± 0.4 for spotted owlet yolk and white. MCF-7 cancer cell lines showed IC50 values of 17.75 ± 0.8 & 21.18 ± 0.5 for hen white , yolk and 21.11 ± 0.7 & 12.16 ± 0.6 for spotted owlet egg white and yolk respectively and the IC 50 values of spotted owlet egg white and yolk on HeLa cancer cells are 24.16 ± 0.5 & 35.57 ± 0.6 , Doxorubicin 10.30 ± 0.4. The antiserum against RCP binds to Riboflavin carrier protein and depletes riboflavin in the cancer cell lines which might have lead to cell growth inhibition

To read the full article Peer-reviewed Article PDF image

Author(s): Karunakar Rao Kudle and M P Pratap Rudra

Keywords

Riboflavin Carrier protein (RCP), antibodies (Antisera), HeLa cells, MCF-7 and A549 cancer cells, Cytotoxic activity., cancer cell lines

 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us