alexa Abstract | Detection of antibody and antigen in extrapulmonary tuberculosis patients' sera using a cocktail of mycobacterial excretory secretory antigens and their antibodies

Biomedical Research
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Abstract

Increased incidence of extrapulmonary tuberculosis (EPTB) as co-infection is observed due to rise in human immunodeficiency virus (HIV) infection. However, the precise diagnosis of EPTB is faced with difficulty due to the lack of tissue biopsy or fine-needle aspiration cytology (FNAC) facilities in rural hospitals. Enzyme linked immunosorbent assay (ELISA) will be simple, convenient and doesn’t require sophisticated laboratory. Mycobacterial antigens ES-31, ES-43 and EST-6 antigens were isolated from Mycobacterium tuberculosis (M. tuberculosis) H37Ra bacilli by affinity chromatography. Cocktail of these antigens and their affinity purified antibodies were explored for detection of antibody and antigen by Indirect and Sandwich ELISA respectively. In a preliminary study with bacteriologically confirmed EPTB cases (n=32), assay of antibody/free antigen/immunecomplexed (IC) antigen showed a sensitivity of 100% and specificity of 90%. Based on this study, sera from suspected EPTB patients (n=164) diagnosed by clinical and other laboratory investigations, non-tubercular disease patients (n=75) and healthy controls (n=75) were screened. A sensitivity and specificity of 72% and 91% for antibody detection, 70% and 94% for circulating free antigen and 63% and 98% for circulating IC antigen detection were observed. On combining the positivity of antibody, circulating free and IC-antigen, overall sensitivity of 96% and specificity of 91% were observed in EPTB. Tuberculous antibody detection to cocktail antigen was found to be useful in detection of EPTB. However, circulating free and IC-antigen detection may be a better marker for detection of different groups of EPTB.

To read the full article Peer-reviewed Article PDF image | Peer-reviewed Full Article image

Author(s): Vijay Upadhye Niraj Shende Satish Kumar and B C Harinath

Keywords

Excretory secretory (ES) antigen, cocktail antigen, cocktail antibody, enzyme linked immunosorbent assay (ELISA), extrapulmonary tuberculosis (EPTB)

 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords