alexa Abstract | Detection of ?-lactamase genes, ERIC-PCR typing and phylogenetic groups of ESBL producing quinolone resistant clinical Escherichia coli isolates.

Biomedical Research
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)


Escherichia coli are one of the most important agents in clinical settings. Treatment of E. coli infections are being complicated due to emergence of resistant bacteria which limits the choices of treatment. The extended spectrum of β-lactamase (ESBL) producing and quinolone resistant E. coli isolates (n= 109) have been isolated from various clinical specimens belonging to both out-coming and in-coming patients of the Erciyes University Medical School Hospital of Turkey. Occurrence of genes encoding major type of β-lactamases (blaCTX-M, blaSHV and blaTEM) was investigated by using PCR among the isolates. ERIC-PCR typing was performed on the isolates. Moreover, phylogenetic groups were determined. The blaCTX-M was detected at the highest frequency (93%; 101/109), while the blaSHV and blaTEM were present in 65% (71/109) and 49% (56/109), respectively. Sequencing of β-lactamase genes from several randomly selected isolates revealed that CTX-M-15 is the only CTX-M type enzyme, while there is variation in possessing SHV and TEM types of enzymes. Isolates harboring three bla genes were 32%. ERIC-PCR analyses, based on two different primers, demonstrated 5 to 6 clonal groups for 42 isolates. Phylo-grouping of all isolates was shown that majority of isolates was belonging to B2 (46%) and D (31%) major groups. The detection of genes encoding major β-lactamases (blaCTX-M, blaSHV and blaTEM) was very high in ESBL-producing quinolone resistant E. coli phenotypic isolates. It indicates that the molecular data was congruent with the phenotypic selection results.

To read the full article Peer-reviewed Article PDF image | Peer-reviewed Full Article image

Author(s): Suleyman Durmaz E Banu Buyukunal Bal Munevver Gunaydin Erkan Yula Duygu Percin


E. coli, ERIC-PCR, ESBL, Quinolone

Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us