alexa Determination of 1,7,7-trimethyl-bicyclo(2,2,1)heptan-2-one in a cream pharmaceutical formulation by reversed-phase liquid chromatography | Abstract

Indian Journal of Pharmaceutical Sciences
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Abstract

A reversed-phase liquid chromatographic method for the determination of 1,7,7-trimethyl-bicyclo(2,2,1)heptan-2-one in a cream formulation is developed and validated. The separation was achieved using an isocratic mobile phase, on a Lichrosorb C8 column. The calibration curve is linear (r 2 = 0.9999) from 25-175% of the analytical concentration of 1.0 mg/ml. The mean percent standard deviation values for intra-day and inter-day precision studies were <1%. The recovery ranges 99.80-100.06% from a cream formulation. The method can be used reliably in quality control for the analysis of bulk cream samples and final product release.

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Author(s): GA Shabir TK Bradshaw

Keywords

Method validation, pharmaceutical cream formulation, reversed-phase liquid chromatography, 1,7,7-trimethyl-bicyclo(2,2,1)heptan-2-one

 
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