alexa Abstract | Determination of carbamazepine in rat plasma in the presence of licorice juice by using HPLC and its pharmacokinetic applications

Journal of Chemical and Pharmaceutical Research
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Original Articles Open Access

Abstract

Carbamazepine (CBZ) is the first choice anticonvulsant and mood-stabilizing drug used primarily in the treatment of epilepsy and bipolar disorder. A simple, rapid and accurate method for determination of carbamazepine in rat plasma in presence of licorice has been developed by using High performance liquid chromatography. A mixture of 55 % of Water Contains (1 ml Triethylamine per 1 liter) and 45 % of Acetonitrile of pH of 6 (adjusted with phosphoric acid) was used as a mobile phase, BDS hypersil C18 column (150mm×4.6mm,i.d 5μ) and a flow rate of 1 ml/min were used, the auto-sampler injection volume was 15 μL, and metronidazole benzoate was used as internal standard. The retention time for carbamazepine and metronidazole benzoate were 2.8 and 3.7 minutes, respectively. The method was validated in terms of linearity (R2= 0.9997) for the concentration (80-4800) ng/ml, precision, accuracy, stability and recovery. A and B groups of rats (n = 8 for each group) were used in the preclinical study. The first group (A), carbamazepine was administrated with water. While in group B, licorice was given instead of drinking water and pre-administrated to the rates before giving carbamazepine dose (10mg/kg). Plasma levels of carbamazepine were compared with plasma levels of carbamazepine with licorice. The maximum plasma concentration (Cmax), area under curve (AUC) and time to reach maximum concentration for carbamazepine with water were (4129.66 ng/ml, 31860.95 ng*hr/ml, and 1.00 hr) respectively. They were significantly reduced in presence of licorice juice (2885.53 ng/ml and 15776.70 ng*hr/ml, 0.50 hr), p value <0.05. In conclusion, we advise to avoid the licorice intake with CBZ or Caution should be considered when licorice is given with carbamazepine, since licorice lower plasma level of carbamazepine when pre-administrated

To read the full article Peer-reviewed Article PDF image

Author(s): Mohd Salemaldajeha Eyad Mallaha Riad Awada Wael Abu Dayyiha Feras DarwishElhajjib Israa H Alanic Basmah A Othmana and Tawfiq Arafata

Keywords

Carbamazepine, HPLC, Pharmacokinetic, drug interaction

 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords