alexa Abstract | Determination of ethionamide in pharmaceutical preparations by visible spectrophotometry employing two sulphonphthalein dyes

Journal of Chemical and Pharmaceutical Research
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Original Articles Open Access


Two simple, sensitive, selective, rapid and reproducible visible spectrophotometric methods are presented for the determination of ethionamide (ETM) in bulk and dosage forms by employing two sulphonthalein dyes: bromophenol blue (BPB method) and bromothymol blue (BTB) as chromogenic agents. . The methods are based on the formation of intensely yellow colored ion-pair complexes upon the reaction of the drug with the cited dyes in chloroform medium. The 1:1 (drug:dye) complexes formed were spectrophotometrically measured at 450 nm with both the dyes without involving the extraction step. Optimization of the experimental conditions is described. Beer’s law is obeyed in the concentration ranges of 0.4-10 and 0.5-14 μg mL-1 for BPB method and BTB method , respectively, with corresponding molar absorptivity values of 1.86×104 and 1.39×104 L mol-1 cm-1. The calculated limits detection (LOD) and quantification (LOQ) were 0.03and 0.09 μg mL-1(with BPB) and 0.05 and1.15 (with BTB). The suitability of the methods for the quantitative determination of ETM was proved by validation in accordance with the requirements of the ICH guidelines. The coefficients of variation were <1.5 % for intra-day and <2.0 % interday analysis, and the accuracy expressed as relative error were< 2.0%. The methods were applied to the determination of ETM in tablets and the results agreed well with the label claim and those obtained by the reference method. The accuracy of the methods was ascertained by recovery study via standard-addition procedure. The possible reaction pathway is also discussed

To read the full article Peer-reviewed Article PDF image

Author(s): Nagib Qarah and Kanakapura Basavaiah


Ethionamide, Determination, Dyes, Ion-pair, Spectrophotometry, pharmaceutical preparations

Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

[email protected]

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals


[email protected]

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

[email protected]

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

social_politicalsci[email protected]

1-702-714-7001 Extn: 9042

© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version