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A simple, precise, rapid and accurate HPTLC method has been developed for the simultaneous estimation of Atorvastatin calcium (ATO) and Losartan Potassium (LOS) in bulk and pharmaceutical dosage form. Precoated silica gel 60 F254 plate was used as stationary phase. The separation was carried out using Toulene: Methanol (8:2 v/v) as mobile phase. The densitometric scanning was carried out at 260 nm. The Rf values was found to be 0.15 ± 0.01 for ATO and 0.09 ± 0.01 for LOS. The linearity was obtained in the range 200-1200 and 500-3000 ng/band ng/band with correlation coefficients (r2 = 0.9994) and (r2 = 0.9999) for ATO and LOS. The method was validated for specificity, linearity, accuracy, precision, LOD, LOQ and robustness. The percentage recovery obtained for ATO and LOS were in the range of 99.25-101.5 % and 100.75-101.26 %, respectively. The proposed method was optimized and validated as per the ICH guidelines.
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Author(s): Kolsure AK Chavan BB Chabukswar AR Kuchekar BS
Atorvastatin calcium, Losartan Potassium, HPTLC, Method Validation