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Research Paper Open Access
The present work reports development and validation of a high-performance liquid chromatography diode array detection procedure for the determination of betamethasone and sodium benzoate in pharmaceutical formulation. Effective chromatographic separation of betamethasone and sodium benzoate was achieved using Nova-pack-C18 (150×3.9 mm, 4 µm) column with gradient elution of the mobile phase composed of 50 mM monobasic potassium phosphate buffer (pH 2.9) and acetonitrile. The elution was a three step gradient elution program with 25% acetonitrile and 75% buffer at 0 min further acetonitrile concentration changed linearly to 45% up to 25 min, followed by changing acetonitrile to 25% up to 35 min. The correlation coefficient for betamethasone and sodium benzoate were 0.9999 and 0.9999, respectively. The developed method was statistically validated with regard to specificity, linearity, accuracy (recovery) and precision.