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Original Articles Open Access
This study aims to evaluate the presence of flavonoids in flowers of different Allamanda species and quantify the rutin by high performance liquid chromatography. The gradient method employs a Phenomenex C18 column with methanol, acetonitrile and water acidified with constant flow of 0.8 mL min-1 and photo diode array (PDA) detection at 355 nm. It was possible to detect several flavonoids in the flowers extract, including rutin with Rt 29.6 ± 0.5 min. Good linearity (correlation coefficient >0.999) for calibration curve of standard rutin was achieved. The limits of detection and quantitation were 0.25 and 2 μg mL-1, respectively. The accuracy, in terms of recovery percentage, ranged from 98.18% to 100.9%. The following concentrations of rutin in the ethyl acetate fraction were obtained: A. cathartica 18.46 ± 0.04, A. schottii 24.85 ± 0.08 and A. blanchettii 45.22 ± 0.08 mg 100g of fresh flowers. The Allamanda is an excellent font of flavonoids.
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Author(s): Tiago J Bonominia Carolina Wittkowskia Folvi D Tomczaka Marcelo M Mafraa Pedro A de Mattosa Rosendo A Yunesb Valdir Cechinel FilhoaMarina da S Machadoa Ruth M Lucindaa and Angela Malheiros
Allamanda, flavonoids, rutin, HPLC, validation method., HPLC