alexa Abstract | DEVELOPMENT AND VALIDATION OF HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC AND UV SPECTROPHOTOMETRIC METHOD FOR ESTIMATION OF PEMETREXED DISODIUM IN BULK DRUG AND PHARMACEUTICAL FORMULATION

International Journal of Drug Development and Research
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Abstract

A high-performance liquid chromatographic method and a UV spectrophotometric method for the quantitative determination of Pemetrexed disodium, a highly potent antifolate, in powder for infusion were developed in present work. The parameters linearity, precision, accuracy, robustness, limit of detection and limit of quantitation were studied according to International Conference on Harmonization guidelines. The HPLC was carried out by reversed-phase technique on Kromasil C18 (250 × 4.6 mm, 5 ?m) column with a mobile phase composed of 20 mM dibasic phosphate buffer (adjusted to pH 6.50 with ortho-phosphoric acid) and acetonitrile (88:12; v/v). The samples were prepared in water and the stability of Pemetrexed disodium in aqueous solution at 25°C was studied. The results were satisfactory with good stability after 24 hr at 25°C. UV spectroscopic determination was carried out at an absorption maximum of 225 nm using distilled water as solvent. Statistical analysis by Student’s t - test showed no significant difference between the results obtained by the two methods. The proposed methods are simple, rapid, precise and accurate and can be used for the reliable quantitation of Pemetrexed disodium in pharmaceutical formulation.

To read the full article Peer-reviewed Article PDF image | Peer-reviewed Full Article image

Author(s): Ankit D Patel Shalin K Parikh Dr D J Sen and Dr C N Patel

Keywords

Pemetrexed disodium, HPLC, UV Spectrophotometry, Validation, Student’s t-test, Pharmaceutical formulation

 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

agrifoodaquavet@omicsonline.com

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

clinical_biochem@omicsonline.com

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

business@omicsonline.com

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

chemicaleng_chemistry@omicsonline.com

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

environmentalsci@omicsonline.com

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

engineering@omicsonline.com

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

generalsci_healthcare@omicsonline.com

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

genetics_molbio@omicsonline.com

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

immuno_microbio@omicsonline.com

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

omics@omicsonline.com

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

materialsci@omicsonline.com

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

mathematics_physics@omicsonline.com

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

medical@omicsonline.com

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

neuro_psychology@omicsonline.com

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

pharma@omicsonline.com

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

social_politicalsci@omicsonline.com

1-702-714-7001 Extn: 9042

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords