alexa Abstract | Development and validation of HPTLC method for simultaneous estimation of Ambroxol hydrochloride, Phenylephrine hydrochloride, Chlorpheniramine maleate, Paracetamol and Guaiphenesin in pharmaceutical formulation

Journal of Chemical and Pharmaceutical Research
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Original Articles Open Access

Abstract

A simple, sensitive, accurate, precise and robust high performance thin layer chromatographic method for simultaneous quantification of Ambroxol hydrochloride, Phenylephrine hydrochloride, Chlorpheniramine maleate, Paracetamol and Guaiphenesin in tablet dosage form has been developed and validated. Chromatographic separation was carried out on Merck precoated aluminium plates with silica gel 60 F254 as the stationary phase. It is a double development method in which first optimized mobile phase used was ethyl acetate for Paracetamol and Guaiphenesin. Second mobile phase used was toluene: methanol: glacial acetic acid (1.4:8.3:0.3, v/v/v) for Ambroxol hydrochloride, Phenylephrine hydrochloride, Chlorpheniramine maleate. Detection wavelength was carried out at 277nm in reflectance/absorbance mode. The retardation factors were found to be 0.82±0.02 for Ambroxol hydrochloride, 0.67±0.02 for Phenylephrine hydrochloride, 0.12±0.02 for Chlorpheniramine maleate, 0.63±0.02 for Paracetamol and 0.47±0.02 for Guaiphenesin. Developed method was validated as per ICH Q2 (R1) guideline. Linearity range for AMB was found to be 1000-10000 ng band-1, for PHE 200-2000 ng band-1, for CPM 100-1000 ng band-1, for PARA 100-1000 ng band-1 and that of GUA 500-3000 ng band-1. The tablet dosage form was found to contain 99.41% (w/w) AMB, 99.77% (w/w) PHE, 100.37% (w/w) CPM, 100.98% (w/w) PARA and 100.44% (w/w) GUA.

To read the full article Peer-reviewed Article PDF image

Author(s): Saurabh S Kardile Sachin E Potawale Sagar S Vidhate Arun M Kashid Amol S Bansode Hira Choudhury Titiksh L Devale and Pravin D Pawar

Keywords

Ambroxol hydrochloride, Phenylephrine hydrochloride, Chlorpheniramine maleate, Paracetamol, Guaiphenesin, HPTLC, Validation., Paracetamol

 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords