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Method describes a development and validation of HPTLC method for the estimation of sitagliptin phosphate and simvastatin in bulk and marketed formulation. This employs a precoated silica gel 60 F254 (0.2 mm thickness) on aluminium sheets and mobile phase chloroform: methanol in the ratio of 8:2 v/v, having chamber saturation for 20 min at room temperature. The developing chamber was run up to 8cm. The Rf values were found to be 0.13 and 0.75 for sitagliptin phosphate and simvastatin respectively. The plate was scanned and quantified at 217 nm. The linear detector response was observed between 2000 ng/spot to 7000 ng/spot and 250 ng/spot to 750 ng/spot for sitagliptin phosphate and simvastatin respectively. The method so developed was validated for its accuracy and precision. The LOD and LOQ were found to be 660, 2000 ng/spot and 50, 150 ng/spot, respectively for sitagliptin phosphate and simvastatin .The recovery was carried out by standard addition method. The Average recovery was found to be 92.80 % and 98.01 % for sitagliptin phosphate and simvastatin respectively.
High Performance Thin layer chromatography, Method validation, sitagliptin phosphate (SITA) and simvastatin (SIMVA)