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Research Article Open Access
A simple, sensitive, reliable and rapid HPTLC method has been developed for the determination of paracetamol, aceclofenac and rabeprazole in combined tablet dosage form. Determination were performed on aluminium backed silica gel 60F254 washed with methanol. The mobile phase used is ethyl acetate- methnol- glacial acetic acid (9: 1: 0.1). The spots were scanned at 275nm. The linearity of paracetamol, aceclofenac and rabeprazole was found to be 100-500μg/ml, 20- 100μg/ml, 2-10μg/ml respectively. The method was validated for accuracy, precision, repeatability. The method was used for the determination of the compound in commercial pharmaceutical dosage forms.
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Author(s): J Bagyalakshmi Sajna John and T K Ravi
HPTLC, pharmaceutical dosage form, paracetamol aceclofenac and rabeprazole, validation.