alexa Abstract | Development and validation of stability indicating method for the quantitative determination of doxorubicin hydrochloride and its related impurities in pharmaceutical dosage forms using RP-HPLC

Journal of Chemical and Pharmaceutical Research
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Original Articles Open Access

Abstract

A simple, linear, rapid, precise and stability-indicating analytical method was developed for the estimation of related substances and degradants of Doxorubicin hydrochloride API and tablets. The chromatographic separations were achieved using high performance liquid chromatograph (X-Terra C18, 100mm X 4.6mm, ID with 3.5 microns particles column) employing mixture of 0.05mM ammonium acetate, methanol and Acetonitrile (500:250 :250v/v) as mobile phase in an isocratic elution mode with a 0.5 mL/min flow rate was chosen. Two impurities were eluted within ten minutes of run time. The column temperature was maintained at 25ºC and a detector wavelength of 254 nm was employed. Doxorubicin HCl was exposed to acid, base thermal, photolytic, and oxidative stress conditions. The stressed samples were analyzed by the proposed method. The degradation of Doxorubicin hydrochloride was observed under Acid hydrolysis, base hydrolysis and oxidative hydrolysis. The drug was found to be stable in all other stress conditions applied. Successful separation of the drug from organic impurities and degradation products formed under forced degradation was achieved. The developed HPLC method to determine the related substances and assay of Doxorubicin HCl can be used to evaluate the quality of regular production samples. It can be also used to test the stability samples of Doxorubicin HCl.

To read the full article Peer-reviewed Article PDF image

Author(s): Sanjeeva Reddy Kallam J Srikanth and K Vanitha Prakash

Keywords

Reverse Phase Liquid Chromatography, Doxorubicin hydrochloride, Validation, Stress conditions, Degradation Products., doxorubicin

 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

[email protected]

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

[email protected]

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001 Extn: 9042

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords