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Research Article Open Access
A simple, accurate, precise and sensitive UV/visible spectrophotometric method were developed for the determination of Rifapentine in bulk and pharmaceutical dosage form. The solvent used was methanol and the wavelength corresponding to maximum absorbance of the drug was found at 478nm. Drug obey’s Beers law in the concentration range of 10- 60ug/ml with correlation coefficient 0.9997. The linear regression equation obtained by least square regression method was y=0.0107x-0.0333, where y is the absorbance and x is the concentration of the pure drug solution. The method was validated for several parameters like accuracy, precision as per ICH guidelines. The values of relative standard deviation and % recovery were found to be satisfactory, indicating that the proposed method is precise and accurate and hence can be used for the routine analysis of Rifapentine in bulk and pharmaceutical formulation.
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Author(s): Ansari Tahir Kalkotwar RS Jeevan Naikwade amp Afsar Shaikh
Rifapentine, Spectrophotometer, Methanol, Method validation., Phytomedicine, Therapeutics