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Tramadol hydrochloride in presence of acidic medium reacts with excess amount of Chloramine-T and remaining Chloramine-T react with Crystal violet to produce blue colour. The final stock solution was made to produce 100μg/ml with methanol. The λmax was found to be 591 nm for assay. The linearity was found in concentration range of 5-30μg/ml. The correlation coefficient was found 0.999. The regression equation was found as Y= 0.025X + 0.001.The method was validated according to ICH Guidelines.
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Author(s): Sagarbechara Padmavathi P Prabhu Subrahmanyam EVS Jignesh J kaneria Sumit saparia Shabaraya AR
Tramadol Hydrochloride, Crystal violet, Chloramine T, H2SO4