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Tizanidine Hydrochloride in presence of acidic medium reacts with excess amount of potassium bromide-bromate and unreacted potassium bromidebromate react with malachite green to produce green colour.The final stock solution was made to produce 100μg/ml with distilled water. The λmaxwas found to be 617nm for assay. The linearity was found in concentration range of 10-30μg/ml. The correlation coefficient was found 0.999. The regression equation was found as Y=0.009x + 0.002. The method was validated according to ICH Guidelines.
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Author(s): Sumit Saparia Padmavathi P Prabhu Dr EVS Subrahmanyam Jignesh Kaneria Sagar Bechara Dr ARShabaraya
Tizanidine Hydrochloride, malachite green, potassium bromidebromate, HCl.