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Original Articles Open Access
Use of polyvalent antisera of equine has been existing as a proven treatment against snake envenomation. However it was found to be low yielding and laborious and cause many side effects. Anti-venom produced from chicken egg yolk serve an ideal alternative source to mammals, as IgY chicken’s blood is transferred to the egg and accumulates in the egg yolk in large quantities. It is a safer, convenient and inexpensive method. The yolk of eggs laid by immunized chicken has been recognized as an excellent source of polyclonal antibodies. 5 month old Indian reds were immunized with cobra venom on day 0 and boosters will be given on day 14 and day 21.The presence of antibody were checked by test bleeding and thereafter eggs were collected and stored at 4°C. Then the antibodies were extracted from egg yolk by Ammonium sulphate precipitation method. The amount of antibody produced was measured using ELISA. The potency of the produced chicken IgY antibodies was tested by neutralization studies.
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Author(s): R S A Sorna Kumar Susan Peter Sangeetha M Sridevi Gnaniah Amuthavalli and Premavathi
Anti-snake venom, cobra, chicken, egg yolk, chicken IgY antibodies, neutralization