alexa Abstract | Development of validated HPTLC method for quantification of oleanolic acid from bark of Madhuca longifolia

Journal of Chemical and Pharmaceutical Research
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Original Articles Open Access

Abstract

This study presents the report of TLC densitometric method, which has been developed and validated for quantification of Oleanolic acid from hydroalcoholic extract of bark of Madhuca longifolia. The separation was performed on TLC aluminum plates precoated with silica gel 60 F254. Good separation was achieved in mobile phase using Toluene: acetic acid: formic acid (9:4:0.5). Determination and quantitation were performed by densitometric scanning at 560 nm in reflection/ absorbance mode. This method gave compact spots at Rf 0.53 corresponding to oleanolic acid. The method was validated using ICH guidelines in terms of precision, repeatability and accuracy. Linearity range for oleanolic acid was 100–600 ng/spot with correlation coefficient R2 ±SD = 0.990 ±5.05% in the concentration range 100–600 ng/spot. The LOD and LOQ were found to be 9.302 and 28.1 ng, respectively. The content in crude extract obtained from bark was found to be 0.418± 0.005% w/w. HPTLC method was found to be simple, precise, accurate and convenient method for rapid screening of active constituents present in hydro-alcoholic extract of plant and can be used for analysis and routine quality control of herbal material and formulations containing Madhuca longifolia.

To read the full article Peer-reviewed Article PDF image

Author(s): Patel V S Jivani N P Patel S B Patel K G Panchal H S and Chhalotiya U

Keywords

Madhuca longifolia, Triterpenoid, HPTLC, HPTLC method

 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

[email protected]

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

[email protected]

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001 Extn: 9042

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords