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Improved differential display of mRNA was used to analyze alterations in gene expression during the internode elongation of basal stem of wheat plant. Three patterns of differential expression were observed: (I) bands observed in pre-elongation basal stem tissue, but not in elongated; (II) bands occurring in elongated but not in pre-elongation basal stem tissue; (III) bands vibrated during the phase. Three differentially expressed cDNA fragments (WSR1-3) had been cloned and sequenced. Sequence homology search in GenBank database showed that one (WSR2) of them has no homology hits; one transcripts (WSR1) had similarity to barley serine/threonine protein kinase; while the product of WSR3 showed high similarity to Ty3/gypsy-type retrotransposon reverse transcriptase, which indicated diverse pathways may be involved in the internode elongation of wheat stem.
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Author(s): Yongchun Li Fanrong Meng Jiangping Ren Jun Yin Hongbin Niu
Wheat, Internode elongation, Gene expression, Differential display.