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Research Paper Open Access
Solvent extraction methods followed by spectrophotometric determination of glipizide and phenformin hydrochloride are proposed here. These method are based on the coloured dye-complex formation between acridine yellow and glipizide (AY-G) and brom cresol green and phenformin hydrochloride (BCG-PH) and their extraction by chloroform. The coloured dye complex of AY-G and BCG-PH in chloroform reveal absorption maximum at 426 nm and 415 nm respectively. The System conforms to Beer's law in the range 15 to 100 Âµg/ml for glipizide and 4 to 24 Âµg/ml for phenformin hydrochloride. Glipizide and phenformin hydrochloride were determined in pure form first and then in oral hypoglycaemic tablets by proposed methods.
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Author(s): P N Phalke A V Sherikar P M Dhadke