Journal of Parasitic Diseases: Diagnosis and Therapy
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article Open Access


Dot Enzyme Linked Immunosorbent Assay (Dot-ELISA) was standardized and evaluated to detect anti-Oesophagostomum antibodies in sera of sheep by using three different antigenic components obtained from the parasite Oesophagostomum i.e., Crude Somatic Antigen (CSA), Excretory Secretory Antigen (ESA) and Larval Antigen (LA). Dot ELISA was standardized at an antigenic concentrations of 0.06 μg CSA, 0.3 μg of ESA and 0.1 μg of LA with sera at 1:100 dilution with Phosphate buffered saline with Tween-20 (0.1% PBST), using sheep anti-rabbit antibodies with horse radish peroxidase enzyme as conjugate at 1:5000 concentration and di-amino benzedine as substrate. The sensitivity, specificity of test were 73.33%, 66.67% by using CSA; 80%, 72.22% by using ESA and as 40%, 66.67% by using LA with statistically significant p value (p<0.05). By using this test 22.01, 10.02, 5.5 were percentage positives detected respective to their antigens, crude somatic antigen, excretory secretary antigen and larval antigen.

To read the full article Peer-reviewed Article PDF image | Peer-reviewed Full Article image

Author(s): Rajeshwari Gaddam Murthy GSS Sudhakar Kommu


Dot-ELISA, ovine oesophagostomosis, excretory secretary antigens

Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version