alexa Abstract | Effect of Aqueous Extract of Roselle Calyx (Hibiscus sabdariffa Linn) on Hidrogen Peroxide Induced Oxidative Stress of Rat Red Blood Cell Membranes

International Journal of Collaborative Research on Internal Medicine & Public Health
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Abstract

Hisbiscus sabdariffa Linn or Roselle is traditional herbs are widely used to treat various diseases. Roselle is rich with natural antioxidant agents such as anthocyanins, vitamin C, protocatechuic acid (PCA) and others. High oxygen reactive species (ROS) such as hidrogen peroxide (H2O2) cause oxidative stress on rat red blood cell (RBC) membrane and lead to hemolysis. The purpose of this in vitro study is to evaluate the effect of aqueous extract calyx Roselle pre-treated on rats RBC membrane towards oxidative stress induced by H2O2. Aqueous extract of calyx Roselle are used on three difference doses of concentration of 0.5, 1.0 and 2.0 mg/ml. The result showed that aqueous extract of calyx Roselle at 0.5 mg/ml concentration as a screening dose was the safest dose based on the reduction about 1.21% of hemolysis. The value was almost near to 1.19% of 0.9% NaCl group (negative control). Furthermore, at dose of 0.5 mg/ml aqueous extract of calyx Roselle studied revealed about 53% reduction via RBC percentage hemolysis when induced by 10 mM H2O2. The study was also measured the concentration of malondialdehyde (MDA), glutathione (GSH) and superoxide dismutase (SOD) activity towards 10 mM H2O2 induction. MDA concentration and SOD activity shows significantly increase as well as the decrease of GSH activity at p value <0.001 compare to 0.9% NaCl control group. Meanwhile, the study on pre-treated RBC with 0.5 mg/ml aqueous extract of calyx Roselle indicated reduction of MDA concentration and SOD activity significantly reduced (p<0.05) whereas GSH activity also significantly increase (p<0.05) compare with control group. In conclusion, this study found that concentration of dose 0.5 mg/ml aqueous extract of calyx Roselle provide a protective effect against oxidative stress induced damage on RBC membrane by 10 mM H2O2 induction.

To read the full article Peer-reviewed Article PDF image | Peer-reviewed Full Article image

Author(s): Jamaludin Mohamed Nor Anizah Mohd Nor Siti Balkis Budin

Keywords

Reactive oxygen species, Hibiscuss sabdariffa Linn, trditional herbs, Malaysia

 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords