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Short Communication Open Access
The effect of cholesterol on vesicle size during freezing and thawing process of extruded egg phosphatidylcholine liposomes were studied by varying the length and number of freeze-thaw cycles. Laser diffraction particle size analysis showed that the volume median diameter of freezethawed egg phosphatidylcholine multilamellar vesicles was increased when cholesterol was included in the bilayers. Using a freeze-thaw cycle of 3 min freezing in liquid nitrogen at -196o followed by 3 min thawing at 50Â° resulted in an anomalously large particle size for egg phosphatidycholine/cholesterol formulations. When egg phosphatidylcholinelcholesterol multilamellar vesicles were repeatedly freeze-thawed, the maximum size was achieved after five freeze-thaw cycles.
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Author(s): S Agarwal G Subramanian P Shetiya N Udupa