alexa Abstract | Effect of stabilizers on stabilization of HBSAG loaded PLGA microparticles

Journal of Chemical and Pharmaceutical Research
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Original Articles Open Access

Abstract

The aim of this study was to prepare poly(lactic-co-glycolic acid) (PLGA) microspheres containing hepatitis B surface antigen (HBsAg) using different stabilizer such as Sucrose, Trehalose, Bovine serum albumin (BSA), Human serum albumin (HSA), Hydroxypropyl-b-Cyclodextrin (HP-b-CD) and combination of Trehalose and HP-b-CD. Suitable stabilizer is required in the PLGA based formulation containing HBsAg to protect the HBsAg antigenicity loss due to usage of organic solvents, release of acids from PLGA and lyophilization. The developed formulations were characterized for entrapment efficiency, antigen structural integrity, Invitro release study, surface morphology and particle size. The entrapment efficiency observed more with trehalose, HP-b-CD and its combination. There is no major structural antigen integrity by SDS-PAGE for formulations with different stabilizers. The in vitro release test showed that combination of Trehalose and HP-b-CD in both primary and secondary emulsion shows improved antigen recovery to >90% on 42 days release as compared to other formulation. Based on these findings, the HBsA with trehalose and HP-b-CD loaded PLGA microspheres could be an effective carrier delivery system for HBsAg

To read the full article Peer-reviewed Article PDF image

Author(s): M Ramvikas K S Jaganathan and Mohamed Ashraf Ali

Keywords

Stabilizers, Trehalose, Hydroxypropyl-b-Cyclodextrin, Hepatitis-B surface antigen, PLGA microparticles, PLGA microparticles

 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords