alexa Abstract | Electrochemical and chromatographic studies of clopidogrel using cathodic stripping voltammetry and HPLC under new experimental conditions and its determination in the preparation tablet, urine and plasma samples

Journal of Chemical and Pharmaceutical Research
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The voltammetric behavior of clopidogrel (CLP) drug have been studied by a cathodic stripping voltammetry technique in the carbonate buffer pH 10 on a surface of hanging mercury drop working electrode (HMDE) νs Ag/AgCl reference electrode and Pt auxiliary electrode. The experimental parameters of cathodic stripping voltammetry (CSV) have been studied and optimized; such as accumulation time and potential, scan rate, pulse amplitude and the convection rate for the analyzed CLP. The high performance liquid chromatographic (HPLC) technique was applied for the determination of CLP using acetonitrile, water and methanol as mobile phase and C- 18 (5μm) with UV detector at 254nm. The sensitivity, accuracy, precision and selectivity of the used techniques were also evaluated. The used analytical methods were developed to determine CLP in the pharmaceutical and biological samples under the typical and optimum conditions. The statistical parameter (F-test) was calculated, which shown an insignificant systematic error between the CSV and HPLC methods.

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Author(s): Ali F Alghamdi


Clopidogrel, cathodic stripping voltammetry, HPLC, biological fluids, HMDE, voltammetry

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