alexa Abstract | Establishment of acute lung injury model in neonatal SD rats

Biomedical Research
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article Open Access

Abstract

Aim: The study is to understand histopathological abnormalities in neonatal Sprague-Dawley (SD) rats with acute lung injury (ALI) induced by different doses of lipopolysaccharide (LPS) and to explore suitable doses of LPS to develop ALI model of neonatal SD rats in 12-48 hours (h).

Methods: (1) 180 neonatal SD rats were randomly assigned into one of six groups: LPS 1mg group, LPS 1.5 mg group, LPS 2 mg group, LPS 2.5 mg group, LPS 3 mg group and control group (n=30 for each group). The rats were inoculated intraperitoneally with 1, 1.5, 2, 2.5, 3 mg/kg LPS and NS. We observed survival rate in each group within 48h. (2) 72 newly-born SD rats were randomly divided into LPS and control groups according to the above method (n=12 for each group). Each group rats were killed at 24, 36, and 48 h. Lungs were collected and used for histological analysis, histological score, determination of wet-dry ratio and radial alveolar cunt (RAC).

Results: (1) The rats in the control group alive all the time. However, the 24-36 h survival rate in the LPS groups were 67 %, 40 %, 23 %, 10 % and 0 respectively; The 48 h survival rate were 33.3%, 6.7%, 0, 0 and 0. (2) LPS aggravated degrees of ALI in neonatal SD rats in a dose-dependent manner. With increasing the dose of LPS and prolonged time, histological score and the ratio of W/D were increased, RAC count was decreased.

Conclusion: The 2, 2.5 mg/kg of LPS induced ALI model of neonatal SD rats might be used to study for the ALI within 24 h. The 1, 1.5 mg/kg of LPS induced ALI model of neonatal SD rats might be used to study for the ALI in 24-36 h. The 1 mg/kg of LPS induced ALI model of neonatal SD rats might be used to study for the ALI in 36-48 h.

To read the full article Peer-reviewed Article PDF image | Peer-reviewed Full Article image

Author(s): Yingying Liu Shaobing Li Jinhui Hu Rong Wu

Keywords

Acute lung injury, Animal model, Neonatal rat, Lipopolysaccharide, #

 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us