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Research Paper Open Access
A sensitive ELISA method for estimation of folic acid in multivitamin formulations has been developed. The assay relies of simple sample preparation step followed by dilution of extracted sample. The detection limit of the assay is 0.1 pg/well. Intra- and interassay variation ranged between 2.7 to 5.1%. Analytical recoveries obtained after spiking with different amount of folic acid ranged between 94 to 104%. The present assay procedure is accurate, selective and useful for direct measurement of folic acid in multivitamin formulation.
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Author(s): J Das Sharma C Duttagupta E Ali T K Dhar