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Research Paper Open Access
The purpose of the investigation was to develop a high performance liquid chromatography assay procedure for quantitative estimation of verapamil in guinea pig serum. The procedure employs less sample volume, simple mobile phase, UV detector and readily available internal standard i.e. propranolol. The procedure is sensitive at lower blood concentrations and permits rapid analysis of upto five samples per hour. In conclusion, a specific and sensitive method has been developed for verapamil assay in guinea pig serum using Î¼ bondapak C18 column and UV detector, which has an added advantage over, published methods.
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Author(s): D D Verma G K Jain S S Agrawal