alexa Abstract | Evaluating the Impact of Boosting on-site Enzymes produced by Trichoderma reesei RUT C30 and Aspergillus saccharolyticus with Commercial Enzymes in lowering the use of Commercial Cellulases.

Molecular Enzymology and Drug Targets
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Research Article Open Access

Abstract

In-house production of cellulases from filamentous fungi is widely used, but their hydrolytic efficiency compared to commercial enzymes is limited. We studied the effect of supplementing in-house cellulases produced by Trichoderma reesei, RUT C30 and a novel strain, Aspergillus saccharolyticus with different types of commercial enzymes for the efficient hydrolysis of wet exploded loblolly pine. Cellic®Ctec 2, Cellic®Htec2 and Novozym 188 were used as the commercial base enzymes for supplementing the in-house produced enzymes. Compared to non- supplemented in-house enzymes preparation, commercial enzymes (Cellic®Ctec2) added in the same amount as FPU and CBU, resulted in 68% higher glucose yield using wet exploded loblolly pine (WELP) at a 20% DM concetration. The highest saccharifcation yield was achieved by supplementation of the in-house produced cellulases with Cellic®Htec2 compared to Cellic®Ctec2 and Novozym 188. Optimal glucose, xylose and mannose yields, 85%, 92% and 86%, respectively were achieved by using in-house enzymes (15 FPU/g cellulose) supplemented with commercial hemicellulase (7.5 FPU/g cellulose). These results showed that supplementing in-house enzymes with commercial enzymes can be advantageous and work for lowering the overall cost of enzymes in a biorefinery.

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Author(s): Vandana Rana Birgitte K Ahring

Keywords

Wet explosion, loblolly pine, , , Cellic®Ctec2, Novozym 188 Introduction Efficient conversion of lignocellulosic polysaccharides to fermentable sugars is the key for commercial production of biofuels [1,2]. Despite many technological improvements, pretreatment and utilization of commercially available lignocellulolytic enzymes to break the recalcitrant nature of lignocellulosic biomass is limiting the viability of producing biofuels [3-7]. Efficient and economically viable ethanol production requires that all sugars produced from cellulose and hemicellulose be converted to ethanol or other bioproducts [8,9]. Enzymatic hydrolysis plays a pivotal role in efficient conversion of the cellulose and hemicelluloses fractions of pretreated biomass materials. The efficiency of enzymatic hydrolysis depends on the pretreatment and right kind and proportions of enzyme cocktails that are used. Numerous studies to investigate the potential of softwood conversion to ethanol have been done in the last two decades [6,10,11]. Pretreatment conditions affect the requisite enzyme mixture employed for polysaccharide hydrolysis. Previously, acid and SO2 catalyzed steam explosion was extensively used to pretreat softwood for enhancing the enzymatic hydrolysis [12- 17]. Among different pretreatment methods developed to date, wet explosion (WEx) is considered one of the most appropriate and cost effective methods for deconstruction of softwood and Trichoderma reesei Aspergillus,saccharolyticus, Enzymes and Drug Targets, Wet explosion

 
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