alexa Abstract | Expression and immunogenicity of H glycoprotein gene of canine distemper virus cloned in eukaryotic expression vector

Journal of Chemical and Pharmaceutical Research
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Original Articles Open Access

Abstract

Canine distemper virus H gene is immunogenic and induces neutralizing antibodies against Canine distemper virus (CDV). In the present study the recombinant plasmid pSin.cdv.h transfected HeLa cell expressed H protein which was confirmed by immunoperoxidase test. The in vivo immunological response was studied in dogs immunized through IM route. The immune response on 30th day post vaccination induced by 4μg dose of rplasmid revealed protective serum neutralizing antibody titer 120, while control group did not show any antibody titer against CDV. Stimulation Index (SI) was found higher in vaccinated group as compared to healthy control animals. The T-cell response in the blood of vaccinated dogs suggested the role of cell mediated immunity in protection apart from neutralizing antibodies in serum. The challenge test revealed 100% protection of vaccinated dogs while all control dogs died of cdv infection. Therefore, this study demonstrates that DNA vaccination with 4μg dose of pSin.cdv.h can provide protective immunity 30 days post vaccination.

To read the full article Peer-reviewed Article PDF image

Author(s): Nitin Sharma Usha Tiwari Manoj Kalakoti Neha K Mehra and Pragya Pandey

Keywords

Canine distemper virus, L.B. medium, E.coli (DH5α) strain, Ampicilline antibiotic, Dogs, glycoprotein

 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords