alexa Abstract | Gender identification of mouse embryos using the Polymerase Chain Reaction (PCR) technique

Biomedical Research
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Abstract

A multiplex nested PCR technique was used to identify gender from single blastomeres biopsied from 8-cell mouse embryos. The primers amplified sequences of the Sry and Zfy genes on the Y-chromosome, and a polymorphic X chromosome microsatellite locus (DXNds-3). Amplification of male DNA gave three bands, of sizes 217 bp (Zfy), 147 bp (Sry) and 111 bp (Nds). In contrast, amplification of female DNA gave a single band of 111 bp (Nds). Test on blood DNA showed a 98.0% PCR amplification efficiency whereas single blastomeres produced 93.0% PCR amplification efficiency. In conclusion, mouse embryos were successfully sexed within 6 hours by using single blastomeres obtained from 8-cell embryos.

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Author(s): Nor Ashikin MNK Abdullah RB Zulqarnain Mohamed Nor Shahida AR Froemming GAR and Mohamed Said MS

Keywords

Embryo biopsy, embryo sexing, gender identification

 
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