alexa Abstract | Gender identification of mouse embryos using the Polymerase Chain Reaction (PCR) technique

Biomedical Research
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)


A multiplex nested PCR technique was used to identify gender from single blastomeres biopsied from 8-cell mouse embryos. The primers amplified sequences of the Sry and Zfy genes on the Y-chromosome, and a polymorphic X chromosome microsatellite locus (DXNds-3). Amplification of male DNA gave three bands, of sizes 217 bp (Zfy), 147 bp (Sry) and 111 bp (Nds). In contrast, amplification of female DNA gave a single band of 111 bp (Nds). Test on blood DNA showed a 98.0% PCR amplification efficiency whereas single blastomeres produced 93.0% PCR amplification efficiency. In conclusion, mouse embryos were successfully sexed within 6 hours by using single blastomeres obtained from 8-cell embryos.

To read the full article Peer-reviewed Article PDF image | Peer-reviewed Full Article image

Author(s): Nor Ashikin MNK Abdullah RB Zulqarnain Mohamed Nor Shahida AR Froemming GAR and Mohamed Said MS


Embryo biopsy, embryo sexing, gender identification

Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version