alexa Abstract | Genome study of North-East Indian Rubus alceifolius

Advances in Applied Science Research
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article Open Access


An attempt was made to isolate genomic DNA along with the estimation of nuclear DNA content of North-East Indian Rubus alceifolius. A simple, efficient and reliable CTAB method is standardized for isolation of genomic DNA from fresh young leaves of R. alcifolius after modifying some of the key steps of earlier protocol described by Khanuja et al. (1999). The modified procedure yielded a high amount (38 μg g-1 fresh leaf tissue) of good quality DNA free from contaminants and found to be suitable for restriction digestion with Eco RI and Hind III enzymes. The nuclear DNA content of R. alceifolius was estimated by one step DNA flow cytometry procedure (Otto, 1990) with slight modifications. The 2C DNA content of this species was estimated to be 5.681 pg. This is the first study attempted for determination of nuclear DNA content of North-East Indian R. alceifolius.

To read the full article Peer-reviewed Article PDF image

Author(s): Jitu Buragohain


Rubus alceifolius, Genomic DNA, nuclear DNA, CTAB protocol, Otto buffer, flow cytometry., Rubus alceifolius

Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version