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Identification of fungi by morphological traits alone is often difficult because mycelial pigmentation, shape and size of conidia are unstable and highly dependent on composition of media and environmental conditions. Molecular techniques such as Internal Transcribed Spacer (ITS) ribosomal DNA (rDNA) have been reliable and are highly suitable tool for identifying fungal species and for assessing genetic variation within populations. The correct identification of fungal isolates from infected eye will help to develop drug against those microbes and also to avoid the possible source of such infections. From the ocular sample collected from infected patients, Cochliobolus sp was identified by basic culture techniques and its genomic profile was evaluated ITS r DNA technology.
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Author(s): J Sumitha C Padmalatha AJA Ranjit SinghP Vanitha Pappa
Internal Transcribed spacer (ITS), Cochliobolus sp, Genomic tools, Molecular identification., physiology, cell and molecular biology, genetics, biochemistry, biotechnology, bioinformatics, microbiology, immunology, parasitology