alexa Abstract | Genotypic distribution of hepatitis C virus among hospital based general population in Vijaypur, India

Journal of Chemical and Pharmaceutical Research
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Original Articles Open Access

Abstract

Hepatitis C virus (HCV) has emerged as a leading cause of chronic hepatitis, liver cirrhosis and hepatocellular carcinoma. Genotypes of HCV can vary in pathogenecity and can impact on treatment outcome. To study the prevalence and different genotypes of HCV among hospital based general population in Vijaypur, Karnataka. Study subjects were those attending various Out-Patient Department (OPD) and In-Patient Department (IPD) of Shri B.M. Patil Medical College, Hospital and Research Centre, Vijaypur, Karnataka. 5ml of blood sample was aseptically collected in plain vial from the study subjects. Serum was separated and aliquoted in different vials. Serum was tested for HCV antibodies by Enzyme Linked Immunosorbent Assay (ELISA) and HCV RNA by Reverse Transcription Polymerase Chain Reaction (RT-PCR). Serum sample positive for HCV RNA was subjected to genotyping by RFLP (Restriction Fragment Length Polymorphism) and direct sequencing. Of the 1,200 samples tested, 32 (2.6%) were positive for anti-HCV antibody and 25 (2.1%) were positive for HCV RNA. HCV genotype 1 (68%) was found to be the most predominant type followed by genotype 3 (32%). The subtypes of genotype 1 were 1a and 1b, where as subtypes of genotype 3 were 3a and 3b. In this study, HCV genotype 1 and genotype 3 were found to be the predominant genotypes respectively. For physicians, knowing the genotype of hepatitis C virus is helpful in deciding type and duration of therapy. In addition, knowledge of circulating genotypes could impact on future vaccine formulations.

To read the full article Peer-reviewed Article PDF image

Author(s): Manjunath Parameshwarappa Salmani and Basavaraj V Peerapur

Keywords

Seroprevalence, RT-PCR, RFLP, Sequencing Genotype, Subtype, hepatitis

 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords