alexa Abstract | Growth kinetics and stability assessment of siphoviridae like Campylobacter phages

Journal of Chemical and Pharmaceutical Research
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Abstract

Campylobacter infections pose a severe threat to the poultry ecosystem leading to high economic loss. The antibiotic treatment against these infections is not effective due to the emerging antibiotic resistance exhibited by the bacteria and no vaccines are currently available to prevent this infection. Phage therapy has been suggested as an alternative antimicrobial therapy. The phages were isolated, purified and the concentration was 22 ×1013 PFU (Plaque Forming Units) mL-1. Over a period of 35 days, these phages were found to be stable when stored both in SM buffer and PBS at 4 - 7°C. The stability of phages was also analyzed at temperature and pH ranging from 50 to 90 °C and 2 to 12 respectively. The phage growth parameters such as the latent period, the burst period and the burst size were 30 min, 30 min and 4 PFU per infective center respectively. These phages possess a wide host range. From the AFM analysis, the head diameter and tail length were 140 nm and 248 nm respectively. From the results of genome finger printing analysis and pulse field gel electrophoresis, the genome size was found to be around 20 Kbp. The presence of different phage proteins in the molecular weight range of 15 – 85 KDa were observed by SDSPAGE.

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Author(s): D Angeline Kiruba S Saranya and M Ananthasubramanian

Keywords

Campylobacter, phage characterization, production, stability analysis, Campylobacter phages

 
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