alexa Abstract | High Performance Thin Layer Chromatographic Method For Estimation Of Moxifloxacin In Tablet Dosage Form.

Indian Journal of Pharmaceutical Sciences
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A new simple, sensitive, specific and precise high performance thin layer chromatographic method has been developed for estimation of moxifloxacin in its tablet formulation (400 mg). In this method, standard solutions and sample solution of moxifloxacin were applied on precoated silica gel G60F254 TLC plate and developed using n- butanol:methanol:ammonia (4:4:2 v/v) as mobile phase: Moxifloxacin showed Rf value 0.50±0.03 and plate was scanned and quantified at 295 nm using Camag TLC Scanner 3. The method was validated in terms of linearity (400-1000 ng/spot), precision (intra-day variation 2.0 to 4.4%, inter-day variation 3.7 to 4.6%), accuracy (96.4 to 102.3%) and specificity. The limit of detection and limit of quantification for moxifloxacin were found to be 10 ng/spot and 50 ng/spot, respectively. The method is simple, sensitive, specific and precise and can be used for the routine quality control testing of marketed formulations.

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Author(s): S A Shah I S Rathod B N Suhagia M V Baldaniya

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