alexa Abstract | High pressure liquid chromatographic determination of vindoline, catharanthine, vincaleukoblastine and vincristine in periwinkle leaf

Indian Journal of Pharmaceutical Sciences
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Research Paper Open Access


A HPLC Method has been developed for the quantitation of vindoline, catharanthime, vincaleukoblastine and vincristine in Catharanthus roseus leaf. These alkaloids extracted with 2 percent tartaric acid and benzene were fractionated by pH gradient extractions. Different fractions were subjected to reverse phase HPLC. Analysis of the eight germplasm lines is described.

Author(s): S Mandal ML Maheshwari

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