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The main aim of present study is to provide adequate numbers of cells to appropriate sites for useful cellular replacement to overcome the functional deficits caused by the trimethyltin chloride lesion. Hip-pocampal disorder was induced by the intraperitoneal administration of trimethyltin chloride ( Sigma chemicals, U.S.A) at a single dose of 7.5 mg/kg body weight or two divided doses of 3.75 mg/kg body weight for two days. The Human Amniotic Epithelial cells to be transplanted were isolated from the fe-tal surface of the human placenta obtained from uncomplicated elective caesarian. Using standard co-ordinates human amniotic epithelial cells injected at four sites of hippocampal formation. For histologi-cal confirmations of the lesion and the growth of the transplant in the hippocampus, rat brains were processed and stained after various post operative periods of 7, 15, 30, 60,120 and 150 days. Trimethy-ltin chloride treatment caused a severe reduction in neuronal cell density in the CA1 and CA3 regions. The human amniotic epithelial cell grafts restored cell numbers in the above said regions to the near normal level. The present study concludes that the human amniotic epithelial cells may be used as a suitable donor tissue to alleviate various degenerative diseases in animal model before the clinical trial in humans, who are suffering from various degenerative diseases.
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Author(s): KV Omprakash R Muthusamy B Devanand K Nagaraja Puranik R Ramesh Kumar M Gurushanthaiah
Dentate gyrus, Hippocampus, Human amniotic epithelial cells, Transplantation, Trimethyltin chloride