alexa Impact of riboflavin-UVA-photodynamic inactivation (PDI) (collagen crosslinking technique) on viability, cell cycle phase, apoptosis and proliferation of human corneal endothelial cells | Abstract

Journal of Chemical and Pharmaceutical Research
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Original Articles Open Access

Abstract

The purpose of our study was to determine the impact of riboflavin-UVA photodynamic inactivation (PDI) (Collagen crosslinking technique) on viability, cell cycle phase, apoptosis and proliferation of human corneal endothelial cells (HCECs), in vitro. A HCEC line was cultured in DMEM/Ham's F12 medium supplemented with 5% fetal calf serum. HCECs cultures underwent 370 nm-UVA-light illumination for 4.1 minutes during exposure to 0.05% or 0.1% riboflavin and 20% dextran containing PBS. Twenty-four hours after riboflavin-UVA-PDI, viability was determined by the Alamar blue assay, cell cycle phase and apoptosis of the cells using the APO-DIRECTTM Kit, and two and twenty-four hours after PDI, HCECs proliferation by the BrdU Cell Proliferation Assay Kit. Twenty-four hours after the use of 0.1% riboflavin concentration without illumination and after 0.05% and 0.1% riboflavin-UVA-PDI, HCECs viability decreased significantly (P<0.01 for all) compared to controls. Twenty-four hours following riboflavin-UVA-PDI, the percentage of HCECs at the G1 cell cycle phase decreased significantly using 0.05% or 0.1% riboflavin concentration (P=0.02 and P=0.03), the percentage of HCECs at the G2/M phase increased significantly using 0.05% riboflavin concentration (P=0.03), compared to controls. Two and twenty-four hours after riboflavin-UVA-PDI using 0.05% or 0.1% riboflavin concentration, HCEC proliferation decreased significantly (P=0.02 for all). There was no significant difference in percentage of apoptotic HCECs at any of the treated groups compared to controls 24 hours after riboflavin-UVA-PDI (P=0.10).Crosslinking arrests HCECs at the G2/M phase, decreases viability and proliferation, however does not trigger apoptosis of human corneal endothelial cells in vitro.

To read the full article Peer-reviewed Article PDF image

Author(s): Jiong Wang Tanja StachonXuefei Song Timo Eppig Achim Langenbucher Berthold Seitz and Nra Szentmry

Keywords

Riboflavin, UVA, human corneal endothelial cells, viability, apoptosis

 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

agrifoodaquavet@omicsonline.com

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

clinical_biochem@omicsonline.com

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

business@omicsonline.com

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

chemicaleng_chemistry@omicsonline.com

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

environmentalsci@omicsonline.com

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

engineering@omicsonline.com

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

generalsci_healthcare@omicsonline.com

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

genetics_molbio@omicsonline.com

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

immuno_microbio@omicsonline.com

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

omics@omicsonline.com

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

materialsci@omicsonline.com

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

mathematics_physics@omicsonline.com

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

medical@omicsonline.com

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

neuro_psychology@omicsonline.com

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

pharma@omicsonline.com

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

social_politicalsci@omicsonline.com

1-702-714-7001 Extn: 9042

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version