alexa Abstract | In vitro antioxidant studies of fresh Brassica oleracea and the characterization of its bioactive compounds using Fourier transform infrared spectroscopy (FTIR)

Journal of Chemical and Pharmaceutical Research
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Original Articles Open Access


In the present study methanolic extract of the pulp of plant Brassica oleracea was investigated for the total phenolic content, total flavonoid content and antioxidant activity. Total phenolic content was evaluated by Folin-Ciocalteau method which revealed the presence of very good amount of phenolic content (14.56μg GAE mg-1) in methanol extract. Total flavonoid content was evaluated by aluminium chloride method which revealed the presence of high amount of flavonoid content (22.65μg QE mg-1) in methanol extract. The extract was also analyzed for antioxidant activity by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay and Ferric Reducing Antioxidant Potential (FRAP) assay. In vitro DPPH radical scavenging assay showed potent antioxidant power with IC50 values of 200μl and Ferric reducing antioxidant power for extract (54.16μM/ml) which are comparable to ascorbic acid. The bioactive compounds present in methanolic extracts were characterized by FTIR spectral analysis. The FTIR spectrum showed the presence of alcohols, phenols, alkanes, alkynes, alkyl halides, carboxylic acids and aromatics, aliphatic amines in methanolic extracts. The present study reveals that Brassica oleracea can be used as a potential source of natural antioxidant which may be used to treat various oxidative stress related diseases.

To read the full article Peer-reviewed Article PDF image

Author(s): Sreelekha Lakshmi D and Veronica Shalini J


Brassica oleracea, Folin-Ciocalteau, 1,1-diphenyl-2-picrylhydrazyl(DPPH), Ferric Reducing Antioxidant Potential(FRAP), Fourier Transform Infrared Spectroscopy FTIR, antioxidant studies

Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version