700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ ReadersThis Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)
ABC transporters export clinically-relevant drugs and their over-expression causes multidrug resistance. In order to knock-down ABC transporters, ABCC1 and ABCC2, 13 shRNAs were developed. Four shRNA candidates were tested in vivo using self-complementary adeno-associated virus serotype 8. A strong, specific knock-down of Abbc2 was observed in mice liver, but at the cost of toxicity caused by oversaturation of the RNAi machinery due to high shRNA expression. Subsequent generation of artificial miRNAs showed better efficacy profile. These results demonstrate the feasibility of knocking down Abbc2 via AAV-delivered shRNAs to the liver, and encourage the use of miRNA in further therapeutics development.
To read the full article Peer-reviewed Article PDF
| Peer-reviewed Full Article
Author(s): Florie Borel Richard van Logtenstein Annemart Koornneef Piotr Maczuga Tita Ritsema Harald Petry Sander JH van Deventer Peter LM Jansen Pavlina Konstantinova
shRNA, miRNA, AAV, Abbc1, Abbc2, multidrug resistance, hepatocellular carcinoma, RNAi, Gene Silencing